Before pipetting, it is required to make sure that the pipette, pipette tip and liquid are at the same temperature. In sucking liquid, the pipette must be kept in an upright position and the pipette tip shall be inserted 2-3 mm below the liquid surface. Before sucking the liquid, it is recommended to suck and release the liquid for several times to wet the suction nozzle (especially when it is going to suck liquid with a viscosity or when the density is different from water). At this time, two pipetting methods can be used:
The first is the forward pipetting method. Press the button to the first stop with the thumb and then slowly release the button to the original point(suck the liquid of the fixed volume), and then press the button to the first stop point to drain out the liquid, wait for a while, continue to press the button to the second stop point to blow out the residual liquid, and release the button in the end.