What are the Key Points of Pipette Operation?

Pipette, also known as the single line pipette, is used to accurately remove a certain amount of liquid, is a common glass instrument in the laboratory daily inspection. In the content determination, the quality of pipette and the method of use have a great influence on the accuracy of the final data.

In order to ensure the accuracy of the liquid volume and the reliability of the experimental results, we can pay attention to the following operation points. In this article, ATO online store mainly introduce the use of pipette need to pay attention to the operation steps.

• Check. Before use, first check whether the pipette has been calibrated, can not use the pipette without calibration. Secondly, check whether the pipe mouth and tip are damaged and blocked, if so, it can not be used. Finally, it should be checked whether the pipette is clean. The use of unclean pipette will make the liquid attached to the wall more, which will make the actual volume deviate from the required volume, or introduce new impurities.
• Wash embellish. It is necessary to moisten and wash the liquid before absorption. The moistening and washing process can not treat the pollution caused by the absorption liquid, especially when the pipe wall is not dry and there are water beads, the absorption speed should be fast to ensure that the suction liquid is not reflux, and avoid the dilution of the liquid in the pipe. If there is liquid at the tip of the pipette before washing, apply filter paper to blot, or directly replace a dry and clean pipette. Generally run washing three times can be, according to need to increase the number of run washing.
• Drain the liquid. Suction liquid when the single channel micropipette inserted into the liquid level can not be too deep or too shallow, too shallow may cause suction (suction will not only pollute the ear washing ball, and if the suction is strong acid strong alkali and other toxic reagents, suction may make liquid splash, very dangerous), too deep will make the tube wall adhesion liquid more and affect the accuracy.
• Release of solution. Before releasing the solution, the index finger should be slightly released to adjust the liquid level. If the index finger or the mouth of the tube is wet, the liquid may not be released or the release speed is difficult to control. At this time, it is best to blot the finger and the mouth of the tube thoroughly with filter paper and then re-absorb the liquid. And when the liquid is released, it is necessary to control the outflow speed with the index finger. It is the outflow of the liquid at a constant speed. Some liquid is easy to hang liquid. After discharging the liquid, observe whether there is too much liquid at the tip of the pipette. If there is too much liquid, continue to lean against the wall and rotate gently.

The above brief introduction of pipette operation points, in the actual work we have more need to pay attention to the place. In addition to the need to pay attention to the use of pipette, in the usual inspection operation should be strict with themselves, pay attention to details, to ensure the accuracy and reliability of the experimental results.